Sentence examples for molecules we performed from inspiring English sources

To test whether mES cells heterogeneously express tissue-specific surface proteins like signaling molecules, we performed flow cytometry analysis.

In order to determine the effects of ubiquitination on the localization of class II MHC molecules, we performed confocal microscopy on live bone marrow-derived dendritic cells (BMDCs).

To determine whether T-cell proliferation induced by purified WTA was mediated by MHC class I or II molecules, we performed assays in the presence of blocking antibodies (Abs) and isotype control Abs.

To examine whether the expression of MMPs in the DCN corresponds to enzymatically active molecules, we performed high resolution in situ zymography (ISZ), involving the conversion of a non-fluorescent substrate (gelatin) into a fluorescent product where gelatinolytic activity is present [28].

To assess the possible influence of these molecules, we performed functional experiments with different mAbs.

To determine if CCR2 deficiency affects chemotactic activity of NTHI-induced SLF-derived molecules, we performed migration assays using primary splenocytes of mice.

To elucidate the molecular pathways involved in the immunosuppressive function of allochimeric molecule we performed microarray and quantitative RTPCR analyses of gene expression profile of splenic T cells from untreated, CsA treated, and allochimeric molecule + subtherapeutic dose of CsA treated animals at day 1, 3 and 7 of post transplantation.

For the detection of mAbs binding to different regions of COMP molecule, we performed ELISA with the recombinant COMP fragments.

Since the faint immunoreactivity observed in normal epithelial cells might have resulted from nonspecific binding of the Fc region of the IgG molecule, we performed IHC analysis using purified F ab) fragment of the IgG molecules.

Finally, in order to show that sCTLA-4 is the only serum protein able to bind B7 molecules, we have performed a similar experiment using sera previously deprived of sCTLA-4 proteins as described in Section 2. Controls were the same sera before depletion.

To detect the cell surface signaling molecules in situ, we performed immunocytochemistry analysis.