Sentence examples for modified sequence was from inspiring English sources

The sortase with a modified sequence was synthesized by Genewiz, sub-cloned to a pET21d vector (Novagen), and purified as previously described (Antos et al. 2016).

Integration into the original target sequence DNA in the substrate DNA was significantly reduced when the substrate DNA including modified sequence was mixed.

A reference-guided assembly to this modified sequence was performed for each sample read pool.

The gene was first cloned in 1995 from human lung alveolar epithelial cells, and a modified sequence was published in 2000 [ 14, 15].

Although the optimization of codon usage in ZmDUR3mod was developed for a better expression in yeast, the modified sequence was also used to perform the functional characterization of DUR3 in tobacco protoplasts and A. thaliana, since also in these latter organisms a high GC content might interfere with the translation of the transcripts.

To analyse this region of the gene for methylation, PCR primers specific for the bisulfite modified sequence were designed.

Mutagenesis studies of OR5.24 revealed inversion from positive to negative charge selectivity by some point mutations [ 79], the resulting modified sequence is present in related receptors suggesting they are candidate ORs for charged amino acid olfaction.

Epitope peptides corresponding to original and modified sequences were validated in HLA binding assays and in immunizations of hemophilic E16 mice, DR3 and DR4 mice and DR3 × E16 mice.

The primers for modified sequences were: sense: 5'-TTGATTATTTGGTGGTGTGTGAG-3' (gi:37704377, 832-854) and biotinylated antisense: 5'-TCATCCATCTTACCTCCCTAATTT 3'-TCATCCATCTTACCTCCCTAATTT 3

The modified sequences are searched by rps-BLAST against the "seed" domain sequence and the percentages of coverage are plotted against each amino acid position.

Further investigation of the mature miRNAs at positions +1 to +18 (excluding the 3'-variable sequences) revealed that, although not predominant (∼5%), modified sequences were present in almost all the miRNAs.