RMA - normalized, averaged gene-level signal intensity (log2) values for the unigenes exhibiting specified sequence similarity were used from all the biological as well as technical replicates and averaged further.
However, the fabrication of full-length collagen mimics of specified sequence de novo has been elusive, and applications still rely on material from native tissues.
As macronuclear development progresses, repressive histone modifications that specify sequences to be eliminated are introduced de novo.
As macronuclear development progresses, Piwi-bound small RNA molecules complementary to micronuclear-specific sequences invade the macronuclear anlage and specify sequences which are targeted to heterochromatin formation by de novo lysine methylation of H3K9 and H3K27 and subsequent binding of Spdd1p.
The method used to generate the randomisation sequence was specified in all three trials.
We considered studies that only mentioned using "sealed envelopes" without specifying how the randomization sequence was generated (2 wrist studies and 11 shoulder studies) as not having reported the randomization method and thus were not awarded any points for randomization.
Many studies did not specify how the random sequence was generated and they were estimated as unclear risk of bias; 88.1% of the RCTs (n=126) did not describe allocation concealment.
Genbank-formatted documents were created that secured specified sizes of intergenic sequences that flank the coding sequence in each genome, taking up to the length specified unless a flanking coding sequence was encountered – in which case only intergenic sequence was secured in a truncated file.
Unless specified, DNA sequences were extracted from UCSC assembly (hg18), and k-mer (k = 1,2,3,4,5) content (count/size) was calculated for each region, yielding a total of 1,364 sequence features to represent the underlying sequence information of a particular genomic region.
This exact peptide sequence was found to exist in both mouse and rat protein homologues, and alignments of these sequences found that the specified intron is retained in both organisms, encoding an additional 37 additional amino acids (Figure 2).
