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Sequencing grade modified trypsin was from Promega (Madison, WI).
Sequencing grade modified trypsin was purchased from Promega (Madison, WI, USA).
Sequence grade modified trypsin was purchased from Promega (Madison, WI; Milan, Italy), and N- 1-pyrenyl) iodoacetamide was from Molecular Probes (EugeN- 1-pyrenyl
Briefly, the dye was extracted and the gel pieces were equilibrated with ammonium bicarbonate, then dried, and a solution of porcine modified trypsin was allowed to soak into the gel.
Sequencing grade modified trypsin was from Promega Corp. (Madison, WI, USA).
Sequencing grade modified trypsin was obtained from Promega (Madison, WI, USA).
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Sequencing grade-modified trypsin was from Promega (Madison, WI, USA).
Sequence grade-modified trypsin was purchased from Promega (Charbonnières-les-bains, France), and Luminata™ Western Horseradish peroxidase (HRP) substrate and ReBlot Plus Strong antibody stripping solution were from Millipore (Molsheim, France).
Twenty micrograms of sequencing grade modified trypsin (Promega) was added, pH was adjusted to 8.5 when necessary, and samples were allowed to digest overnight at 37 °C.
Sequencing grade modified trypsin (Promega) was added at an enzyme-to-substrate ratio of 1 25.
Sequencing grade, modified, porcine trypsin was purchased from Promega (Madison, WI).
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