Sentence examples for modified vector was from inspiring English sources

The modified vector was designated pP[RS3]3′M.

This modified vector was then digested with SfiI, agarose gel purified, and used for ligation with the SfiI digested cDNA samples.

Fifty nanograms of the modified vector was transformed in BL21 (DE3) cells and plated on LB agar plates containing 70 μg/mL ampicillin and 50 μg/mL chloramphenicol.

The mutagenicities of (6 R,8 R)- and (6 S -crotonaldehydehyde adductS -crotonaldehyde1, row 18 adducts were ∼5 and ∼6%, respectively, when a single-stranded, site specifically modified vector was replicated in the COS-7 system (76).

The reduced acrolein-mediated N-dG: N-dG interstrand cross-link was site specifically incorporated into a DNA vector, and this modified vector was utilized to transform wild-type E. coli strain and pol II and pol IV deletion mutants (147).

To permit selection against cells in which the entire attB plasmid has been incorporated by illegitimate recombination, we modified our original attB vectors by adding a HSV TK transcription unit, whose product renders cells sensitive to GCV; these modified vectors are shown in Figure 1 and were used in all the remaining experiments described in this paper.

The modified vector pHSG299_CSPS was amplified with two primers, CSPS-N2-CAA-u and CSPS-C2-u (Supplementary Table S1), and digested with two enzymes, SfiI and I- SceI.

An understanding of gene flow and environmental barriers to mosquito dispersal is also essential if plans to genetically modify vector populations are to be realized (Scott et al. 2002; James et al. 2006).

A modified pET28b vector was designed to produce N-terminal polyhistidine-tagged proteins with a simple construction scheme having broad applicability because of the use of rare SapI cloning sites.

The modified pACYC177 vector was designated pACYC177L.

This modified pACYC177 vector was designated as pACYC177L1.