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In the current study, the modified protocol was applied to three different bacterial species (two gram-negative and one gram-positive).
The modified protocol was used for the verification of Chinese Spring ditelocentric stocks before DNA extraction for PCR amplification of triticin gene segments.
Because significant aggregation of hβ2 occurred after 100°C heating, a modified protocol was applied on hβ2 samples: the heating treatment was omitted and the incubation time with enzyme was increased to 24 hours.
This two step modified protocol was independently replicated externally.
The modified protocol was named Restriction and Circularization Aided Rolling Circle Amplification (RCA-RCA).
The specifically modified protocol was used (0 to 1 000 Da, 12 to 17% laser intensity).
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This modified protocol is called Ancestors protocol.
In addition to this, a modified protocol is designed and developed.
According to the comparative results, it found that the modified protocol is always outperforming the ASGK protocol.
The modified protocol is simple to implement since it does not require any change to the network topology and uses only information that is already available in a standard Chord network.
As the modified protocol is also potentially more stressful to the animals, it is concluded that the use of additional induction applications in the Buehler test cannot be justified from either a scientific or an animal welfare perspective.
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