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The modified gene was ligated into pGHost+9 vector and electroporated into the streptococci.
The modified gene was ligated into pGEM-T Easy and verified by sequencing.
Another modified gene was Tspn8, which may exert tumor-promoting activities by increasing cell motility and by inducing angiogenesis [ 26].
The G14 version of this modified gene was the most effective against PTM in transgenic potato [ 17] and therefore used in this study.
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The modified genes are under the control of their native promoters, terminators, and UTRs.
The modified genes were cloned into an S. cerevisiae overexpression vector and transformed into [PSI+] cells.
Around half of all H3K27me3 modified genes were also H3K4me3 modified in both PC3 and EP156T cells, showing that bivalent H3K4me3 and H3K27me3 can be found in both prostate somatic cells and cancer cells.
Further analysis indicated that 825 of the modified genes were 'Su Shuand-specific and 3121 of the modified genes were 'Indo'-specific.
In contrast, 92% of expressed genes were non-H3K27me3 modified, and 80% of H3K27me3 modified genes were silent genes.
The majority of differentially modified genes were derived from ASHM genes.
It was recently described that Bioinformatic analyses of 99 modified genes were associated with human autism.
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