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A diesel engine served as a heat source by providing hot exhaust gas into the DCTEG, while the coolant (water ethylene glycol mixture) was pumped into the coolant channels to remove heat.

This mixture was pumped back and forth between fermenter and post-digester several times during the next 10 days.

Bacterial beads were prepared by adding 10 mg/mL of bacterial biomass into sodium alginate solution and this mixture was pumped drop wise into 0.2 M CaCl2 solution in sterilized condition under laminar flow.

Prior to starting the experiment, the water/inoculum mixture was pumped in circulation over the substrate for 10 min followed by a single step for pH adjustment to an initial value of 5.46 (±0.05) using 1 M NaOH.

The pH of the ammonium acetate was adjusted to 4.0 by addition of NaOH and HCl, and the mixture was pumped at 25 °C with a flow rate of 1.3 mL min−1.

The cell mixture was pumped in the chip at a flow rate of 3 μL min−1 for 1 min from the inlet by a syringe pump, followed by pausing the flow for 3 min till cells were trapped in microwells.

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Step on the throttle and the mixture is pumped past a catalyst made of ruthenium.

Through these risers, a highly viscous concrete mixture is pumped under high pressure into the waiting forms above.

a The cell mixture is pumped into a microfluidic chip which consists of three layers: the microfluidic channel, the cell trapping array and the cell lysate collecting chamber.

To make the balls, a chemical mixture is pumped into molds, which are then baked in ovens.

After mixing the eluate with the RT-PCR buffer (95.25 μL iTaq universal probes one-step buffer and 4.75 μL iScript, both from Bio-Rad, USA), 25-μL aliquots of the mixture were pumped into the PCR wells each containing a primers and probe mixture of one of the biomarkers MMP9, OLFM4, CD177, LCN2 and B2M.

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