Further 10 20% of the same mixture was injected IP as necessary to maintain surgical plane anaesthesia.
cELP was mixed with enfuvirtide and a small amount of hydrogen peroxide (to promote cross-linking), and the soluble mixture was injected subcutaneously.
120 μl of the supernatant was mixed with 30 μl of the internal standard (0.05 M dihydroxybenzylamine) and 125 μl of this mixture was injected into the HPLC system.
Then, the mixture was injected into 800 µl of buffer R (pH 8.3) (20 mM tricine, 20 mM succinic acid, 80 mM NaCl and 0.6 mM KOH) which was pre-supplemented with 100 µl of luciferase/luciferin mix (CLSII).
A suspension with 5×106 cells in 0.1 mL Matrigel mixture was injected subcutaneously into the flank of 8-week-old BALB/c-nu/nu mice (Orient Bio Inc., Gyeonggi-do, Republic of Korea).
This mixture was injected intramuscularly in the femoral quadriceps.
And the BE3 mRNA and gRNA mixture was injected after the appearance of pronucleus.
The mixture was injected into a stainless steel autoclave with a Teflon container cartridge.
And YEE-BE3 mRNA and gRNA mixture was injected after removing PB1.
Mesoglein was cut out from the slab gel, homogenized with 0.9 % NaCl and mixed with Freund adjuvant (1 1); this mixture was injected hypodermically into male Guinea pig.
After brief cooling, reaction mixture was injected into the preparative HPLC system consisting of Luna C18 (2) reversed phase column (Phenomenex, Torrance, CA, USA).
