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The preparation lasted for approximately 190 min followed by a minimum of 30 min of stabilization before measurements.

The IS measurement as a function of RH was carried out after 30 min of stabilization at each measuring RH with step of 5 or 10%%.

A total of 6 mg/kg IV over 5 min was administered followed by 30 min of stabilization before recording baseline measurements.

After 30 min of stabilization, a second set of measurement was recorded, including the same hemodynamic, respiratory, and tissue oxygenation variables (Additional file 1).

For the IS measurements performed as a function of T at constant RH, impedance spectra of hydrated samples were collected after at least 30 min of stabilization at each measuring temperature.

Oxyphor G2, a two-layer glutamate dendrimer of tetra- 4-carboxy-phenyl) benzoporphyrin (Oxygen Entetra- 4-carboxy-phenyllphia, PA, USA), was subsequentetra- 4-carboxy-phenyl/kg IV over 5 min), followed by 30 min of stetra- 4-carboxy-phenyl

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Similar(38)

A blood gas analysis was performed after a 30 min period of stabilization of blood oxygen saturation.

HAP aggregate size was measured in a Nano-ZS from Malvern, at temperatures ranging from 20 to 40 °C, after 5 min of temperature stabilization.

For high VT, ventilation settings were changed from normal VT after 30 min of hemodynamic stabilization, to high VT = 47 ± 2 ml/kg, targeted to a PIP = 35 ± 0.5 cmH2O, RR = 60 breaths/min, PEEP = 1.5 cmH2O, without recruitment maneuvers.

After 30 min of a stabilization period, coronary artery occlusion was induced by inflation of the balloon catheter.

The left ventricle pressure was then monitored after a 10 min-period of stabilization.

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