Exact(2)
The CVB4/p24 733) recombinant was more rapidly inactivated than the parental vector during the first 30 min of inactivation.
A sterilizing rate up to 99.9% could be obtained after 60 min of inactivation.
Similar(58)
The reaction proceeded with 5 min annealing at 25 °C, 60 min at 42 °C and 15 min of heat inactivation at 70 °C.
After 120 min of heat inactivation of topoisomerase II, DNA helical tension accumulated in Δ top1 tsp2 ts Δ sir3 cells expressing the E. coli TopA gene.
The control was run by substituting the enzyme with the same volume of enzyme extract heated in a boiling water bath for 30 min for inactivation of the enzyme.
The tubes were placed in a 100°C block for 10 min for inactivation of the proteinase K. PanINs was classified into four categories of PanIN-1A, -1B, -2 and -3 according to the standardised nomenclature and classification of pancreatic intraepithelial neoplasia (Hruban et al, 2001).
The sample was incubated at 37°C for 4 h followed by incubation at 65°C for 20 min for inactivation of the enzyme.
However, after 120 min of topoisomerase II inactivation, most minichromosome molecules seemed highly positively supercoiled.
After 120 min of topoisomerase II inactivation, relative abundance of transcripts from genes at the chromosomal flanks were not significantly increased, in sharp contrast to that observed on accumulation of DNA helical stress.
We extracted total RNA from the above top2 ts and TOP2 cells after 0, 30, and 120 min of topoisomerase II inactivation and used microarrays to compare transcript levels between both strains.
To examine how transcriptome alterations between the above top2 ts and TOP2 strains spread throughout the yeast chromosomes after the accumulation of DNA helical stress, we plotted the relative transcript variations (after 0, 30 and 120 min of topoisomerase II inactivation) versus the respective gene distance from the telomere.
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