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The test was initiated at 09.30 h, after obtaining at least 3 min of stable BP and HR measurements.
For both extracellular and patch-clamp recordings, three consecutive responses were averaged and at least 10 min of stable baseline recording preceded drug application.
After 15 min of stable recordings, artificial cerebrospinal fluid (aCSF) containing CNO (1 µM solution) was perfused through the chamber and recordings continued for another 5 min before washed out by aCSF27,35.
Measurements were performed after 5 min of stable hemodynamics.
After 10 min of stable baseline response to test stimulation (once every 30 s), the ability to elicit LTP was assessed.
After 20 min of stable baseline recording, LTP was induced by four sets of HFS at 100 Hz with 10 sec interset intervals.
Similar(41)
A 15 min period of stable isometric contraction was imposed prior to the experimental period.
Patients received in randomised order either DA or NE to achieve and maintain a CPP of 70 mmHg and then, following a 30 min period of stable haemodynamics, a CPP of 90 mmHg.
The recording in B is no longer displayed after 90 min of continuous stable XII output following the end of the ablation phase.
After 15 min of a stable baseline response (0.05 Hz frequency stimulation), a tetanic stimulation was applied to induce LTP (four trains of 1 s each, 100 Hz within the train, repeated every 15 s).
Test with single pulse was then resumed for 90 min to determine the level of stable LTP.
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