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To limit differences between subjects in the basal temperature, SBF was recorded at a local skin temperature of 37 °C allowing for at least three minutes of stabilization.
We collected arterial blood gases, respiratory and hemodynamic variables, and mixed expired gases at baseline and after 60 minutes of stabilization at ultraprotective ventilation.
Following 5 minutes of stabilization, every animal received deep lung inflation to 30 cm H2O distending pressure to ensure uniform lung recruitment, and normalization to the same pre-assessment volume history.
The 60-second averages of each test condition were made after 20 minutes of stabilization.
The mesenteric arterioles were allowed 15 minutes of stabilization and pre-constricted with norepinephrine (10-5 mol/L, NE).
Each change in pressure was followed by approximately five minutes of stabilization before the measurements were taken.
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After 60-minutes of stabilization, sublingual microcirculatory images were acquired using a Sidestream-dark Field device (SDF) for ulterior semi-quantitative analysis.
Four mL of Ringer solution was added to each side of the intestinal epithelium and the preparations were allowed a period of 60 minutes for stabilization of the electrical parameters before the start of the experiment.
After a wash (W) and 20 30 minutes period of stabilization, arterial ring responses to endothelin (ET-1) were studied.
Within minutes of the stabilization board's announcement, Jake Brace, United's chief financial officer, held a conference call with union officials, telling them that United did not plan to file for bankruptcy protection immediately, said one official who listened to the call.
Haemodynamic measurements were started after completion of instrumentation and 30 minutes of haemodynamic stabilization.
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