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Six dogs undergoing mild injury received 30 min of occlusion followed by 120 min of reperfusion (IR30).
Seven dogs undergoing severe injury received 120 min of occlusion followed by 120 min of reperfusion (IR120).
Animals were lightly anesthetized to place the microarterial clamps but were awake and spontaneously ventilating during the 10 min of occlusion.
Two CCAs were released after 30 min of occlusion while the distal MCA remained occluded; the CCA release allowed partial reperfusion and reperfusion was not detected in the ischemic core after CCA release.
The concentrations of active TGF-β1 in the extracts obtained after 120 min of occlusion were also higher in WT mice than Thbs1−/− mice (p = 0.041 by t-test).
Levels of active TGF-β1, expressed as a percentage of total TGF-β1 were higher in extracts prepared from thrombi obtained after 120 min of occlusion than after 5 min in WT mice, but not in Thbs1−/− mice [1.7±1.2% after 5 min (n = 5) and 2.6±1.7% after 120 min (n = 5) in WT mice; 1.9±1.6% after 5 min (n = 5) and 2.0±2.3% after 120 min (n = 5) in Thbs1−/− mice] (Fig. 4, filled triangles).
Similar(39)
The cuff was instantaneously deflated after 5 min. of occlusion and during the first 90 s after cuff release reactive hyperemic intensity weighted mean blood velocity signals were obtained as described above, followed by a B-mode image of the left brachial artery for 12 cardiac cycles at a frame rate of 11 frames/second.
To first evaluate whether enhanced neurogenesis and angiogenesis were a consequence of exacerbated brain damage only, we exposed PrP−/− mice to a shorter MCA occlusion of 30 min, whereas WT mice again were submitted to 45 min of MCA occlusion.
We perfused a number of kidneys after a period of 45 min of vascular occlusion.
The IR30 group underwent 30 min of LCx occlusion followed by 120 min of reperfusion followed by Tc-99m-teboroxime administration.
To evaluate the left ventricular remodeling 2 months after reperfusion, 30 min of LCA occlusion followed by reperfusion with (n = 6) and without postconditioning (n = 6) was performed.
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