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A preconcentration factor of 20 and a detection limit (3sb) of 2.1 ng L−1, along with a sampling frequency of 28 h−1 were achieved with 1.4 min of sample loading time and with 2.8 mL sample consumption.
The resulting DNA duplexes could be visualized trapped at the LFD strip test line within 5 min of sample exposure.
Disintegration was performed at 4000 rpm in three cycles consisting of 2 min of disruption and 2 min of sample cooling on ice.
Data on sperm velocity parameters were obtained within 5 min of sample collection for all individuals.
Plasma was separated within 30 min of sample collection by centrifugation at 4°C.
Plasma not required for biochemical analyses was frozen at −20°C until analysis, within 30 min of sample collection.
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Fig. 3 Plot of particle sizes (μm) versus ground time (min) of samples S4, S5, S6, S7, and S8.
We thus measured LDH activity at periods of 1, 2, 4 and 8 h after preparation and observed no increase (15 min of sampling in SIF, n = 3, data not shown).
The reduction of plasma NEFA concentration led to significantly greater glucose clearance rate (1.9 vs. 1.2%/min) and to decreased glucose half-life (37 vs. 58 min), time to reach basal concentration (81 vs. 114 min) and glucose response area under the curve during 180 min of sampling [6,942 vs. 10,085 (μIU/mL) × 180 min].
Enhanced glucose clearance was achieved when plasma NEFA was reduced by NA, despite lower insulin concentration (70.0 vs. 97.9 ± 13.4 μIU/mL) and a tendency for smaller insulin response area under the curve during 180 min of sampling [7,646 vs. 12,104 ± 2,587 (μIU/mL) × 180 min], reflecting an increased response to endogenous insulin.
Plasma samples were immediately put on ice and spun at 4°C within 30 min of sampling.
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minimum of sample
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minute of sample
min of specimen
less of sample
min of random
min of testing
expressions of sample
minutes of sample
min of illumination
min of occlusion
min of sonication
min of audio
min of irradiation
min of injection
min of operation
min of immersion
min of anodization
min of inactivation
min of plasma
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