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A preconcentration factor of 20 and a detection limit (3sb) of 2.1 ng L−1, along with a sampling frequency of 28 h−1 were achieved with 1.4 min of sample loading time and with 2.8 mL sample consumption.

The resulting DNA duplexes could be visualized trapped at the LFD strip test line within 5 min of sample exposure.

Disintegration was performed at 4000 rpm in three cycles consisting of 2 min of disruption and 2 min of sample cooling on ice.

Data on sperm velocity parameters were obtained within 5 min of sample collection for all individuals.

Plasma was separated within 30 min of sample collection by centrifugation at 4°C.

Plasma not required for biochemical analyses was frozen at −20°C until analysis, within 30 min of sample collection.

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Fig. 3 Plot of particle sizes (μm) versus ground time (min) of samples S4, S5, S6, S7, and S8.

We thus measured LDH activity at periods of 1, 2, 4 and 8 h after preparation and observed no increase (15 min of sampling in SIF, n = 3, data not shown).

The reduction of plasma NEFA concentration led to significantly greater glucose clearance rate (1.9 vs. 1.2%/min) and to decreased glucose half-life (37 vs. 58 min), time to reach basal concentration (81 vs. 114 min) and glucose response area under the curve during 180 min of sampling [6,942 vs. 10,085 (μIU/mL) × 180 min].

Enhanced glucose clearance was achieved when plasma NEFA was reduced by NA, despite lower insulin concentration (70.0 vs. 97.9 ± 13.4 μIU/mL) and a tendency for smaller insulin response area under the curve during 180 min of sampling [7,646 vs. 12,104 ± 2,587 (μIU/mL) × 180 min], reflecting an increased response to endogenous insulin.

Plasma samples were immediately put on ice and spun at 4°C within 30 min of sampling.

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