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Preferences were expressed as the proportion of feeding time during the first min of test.
The depressant action was increased with time and a noticeable result was found at 120 min of test sample administration.
All the tests were conducted at the frequency of 25 Hz, amplitude of 1 mm, and 30 min of test duration.
It is matched with the profile of ethanol sol stability level, where it is not relatively constant until 90 min of test, but gradually decreases, which indicates the slow sinking process of titania particles (not an immediate process).
In rats treated with an intragastric load of 2 M NaCl, baclofen injected into the LPBN increased 0.3 M NaCl intake from 150 to 210 min of test and reduced water intake from 30 to 150 min of test.
On the contrary, baclofen into the LPBN reduced water intake in cell-dehydrated rats (30 150 min of test).
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The PIAAC test design (OECD 2013b) assumed 60 min of testing time for the cognitive assessment.
However, approximately 13% of sulfide (i.e., 104 μM out of the original 800 μM) was lost in the system with 3.0 g/L of illite within 20 min, and stabilized afterward during the 120 min of testing period.
Only data from animals that reached the goal box before 30 min of testing were included in the behavioural analyses.
During the 5 min of testing, exploration of an object was defined as pointing the nose to the object and/or touching the object with the nose.
However, when the tone CS came on, the SFC rats immediately displayed freezing and USV that persisted through the entire 8 min of testing (Fig. 2A).
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