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The majority of currently available rapid HIV tests require that the test be conducted within 5 min of specimen collection.
In this connection, it should be noted that polarization curves are built after 15 30 min of specimen exposition in the corrosion environment, when corrosion potential E is stabilized, while the parameters K and P were calculated on the base of corrosion tests duration of 99 288 h.
A morphologic diagnosis may be obtained within 10 min of specimen arrival in the electron microscopic facility.
One such test, Cepheid Xpert CT/NG (Cepheid, Sunnyvale, California, USA), has at least equivalent performance to traditional laboratory-based NAATs, providing results within 90 min of specimen collection.
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Based on prior experience in other fields, a starting sample size (N min ) of 20 specimens was considered large enough to make a classification in many cases.
For endocervical specimens, 1 ml of specimen lysis buffer was added to endocervical samples, mixed thoroughly by vortexing and were incubated for 10 min at room temperature.
Reverse transcriptase was heat inactivated at 94°C for 4 min. Q-PCR of specimen and standard cDNA was completed using TaqMan predeveloped assay reagents.
After 1 hr of incubation at 39°C, the reverse transcriptase was heat inactivated at 94°C for 4 min. Q-PCR of specimen cDNA and standard cDNA was performed using TaqMan master mix (Perkin Elmer, Foster City, CA), 1.25 μM probe, 3 μM forward primer, and 3 μM reverse primer in a 50-μL volume.
After 15 min of storage, the specimens were put into a new centrifuge tube (tube 2, at time point 45 min) with 20 ml fresh saline and the pressure was raised again to 525 hPa.
During spawning induced by KCl injection one or two gonopores released gametes continuously for several minutes (maximum of 30 min with specimen underwater); the flux of gametes through a genital papilla varied between 7 and <img src="http://journals.plos.org/plosone/article/asset?id=info?doi/10.1371/journal.pone.0009654.e012.PNG" class= inline-graphic"/> (Video S1).
Thus, if biochemicals were detected in at least 70%% of each group within a tissue of interest (e.g., at least 6 of the 8 soleus samples), the missing data were imputed with the lowest detectable value (1 × MIN) specific to the type of specimen, and a relative ratio was reported.
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