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Only a gram or less of sample is required with improved assays and the FC is remarkably resilient to degradation for more than a week at room temperature [ 16].
Phi29-based MDA is a highly reliable approach for generating abundant quantities and good quality amplified product from the template DNA without the need for thermal cycling and starting from very small quantities (picograms or even less) of sample DNA [ 2, 15- 18].
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Metastatic LNs were observed in less of samples with LNs (>12) than samples with <12 LNs.
Very less amount of sample is required for processing.
Compared to normal flow injection analysis, the rFIA method is superior due to its lower reagent consumption, less dispersion of sample, as well as higher sensitivity.
This robust prediction performance was powered by the merits of WAI scheme that could provide improved reproducibility by illuminating large sample areas as well as less sensitivity of sample placement by the design of long focal length.
By comparison, protein microarray based-serum profiling is much more sensitive and can be performed at higher throughput while requiring less amount of sample.
Even though less amount of sample is needed for successful on-line BCD than for traditional dereplication processes, in many cases, still substantial amounts are required.
We classified rare CNVs as those occurring in 5% or less of the sample.
The other four health states (B, C, D, F) were rated as negative by 5.5% or less of the sample.
Therefore, we can say that, compared to other methods, the SS algorithm (i) generates better estimations with less number of samples; (ii) provides more accurate results; and (iii) is less sensitive to parameter tuning.
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