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20110073).To evaluate the effects of CAPA on infarct size in healthy rats, the left anterior descending coronary artery (LAD) of 8-week-old rats was occluded for 45 min and reperfused for 2 hours; CAPA and dmCAPA were given intraperitoneally 30 min before reperfusion, while the nitric oxide synthase (NOS) inhibitor was given 15 min before CAPA and dmCAPA administration.
Cooling began 30 min before reperfusion.
These data suggest that morphine and ibuprofen reduce infarct size individually or at subthreshold doses in combination by 12-LO when administered 5 min before reperfusion.
Acute FIT administration reduced IS when given before ischemia, 5 min before reperfusion, or 10 s after reperfusion compared with control.
5-HD administered either before SB21 treatment or 5 min before reperfusion the following day abrogated SB21-induced protection (54 +/- 4% and 61 +/- 2%, respectively).
The MPTP opening either before SB21 administration or 5 min before reperfusion abrogated the infarct size reduction produced by SB21 (61 +/- 2% and 62 +/- 2%, respectively).
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To investigate the cardioprotective effects of CAPA against I/R injury in vivo, rats were intraperitoneally treated with CAPA (3 and 15 mg/kg) or dmCAPA (CAPA derivative, methylation at the hydroxyl groups, no antioxidant activity; 15 mg/kg; Figure 1) 30 min before the reperfusion.
After a 30-min ischemia, but before reperfusion, an intravenous bolus of EPA and DHA (6:1), associated or not with iodinated contrast media, was administered.
In Lodge et al.[ 20], patients received multiple doses of rFVIIa immediately prior to first incision, then every 2 h until 30 min before anticipated liver reperfusion, with an additional rFVIIa dose at the end of surgery.
IPC protocol was 5-min stabilization, 5-min ischemia and 5-min reperfusion before the index ischemic event.
In Group III, subjects underwent three cycles, 5 min each, of ischemia followed by 5 min of reperfusion, before receiving 30 min of ischemia.
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