Sentence examples for min before stimulation from inspiring English sources

For CXCR4 receptor-blocking experiments, AMD3100 (Sigma-Aldrich) or MEK inhibitor U0126 (Promega, Fitchburg, WI) were added to the medium at a final concentration of 10 μM; control cultures received identical volumes of vehicle solution (0.1 M PBS) 30 min before stimulation with CXCL12.

Five percent CO₂ was applied for 5 min, 3 min before stimulation.

PP2 was purchased from Alexis and added to MoDC used 20 min before stimulation with TLR agonists.

After 24 h, cells were incubated in M199 containing 10 mM Hepes and 20 mM LiCl for 15 min before stimulation, then lysed in 0.1 M HCOOH.

This antibody was given 1 min before stimulation with Interleukin-8 (IL-8; Sigma-Aldrich) or and Leukotriene B4 (Larodanarodan Fine Chemicals AB).

Infant HR was recorded continuously during the 5 min before stimulation, during stimulation and the 5 min after stimulation, to see whether behavioral state changed.

The cells were treated with the inhibitors or their vehicle (0.1% vol/vol DMSO) 45 min before and during stimulation with the cytokines for 24 h.

Growth-arrested cells were treated with SP600125 (22.7 μM) or vehicle (0.1% vol/vol DMSO) 45 min before and during cytomix stimulation for 3 h.

Growth-arrested ASM cells were treated with SP600125 (10 μM) or vehicle (0.1% vol/vol DMSO) 45 min before and during cytomix stimulation for 0, 5, 10, 30, and 60 min. Total cell lysates were prepared (19, 29) and, together with prestained standards and JNK controls, subjected to SDS-PAGE gel electrophoresis and Western blotting.

To keep animals quiet during electro-stimulation, the rats were fastened to an animal plate and adapted for 10 min before electroacupuncture stimulation.

Seedling roots were first inverted for 7 min (root tip upward) and then placed in the downward (normal) position for 7 min before gravitropic stimulation in the horizontal position.