Sentence examples for min before incubation from inspiring English sources

The cultured specimens were decontaminated with BD Mycoprep (4% NaOH 1% NLAC and 2.9% sodium citrate) for 10, 15 and 20 min before incubation in Mycobacterium growth incubator tube (MGIT) system and growth examined for acid-fast bacilli (AFB).

Briefly, RNA (in 5 μl) was added to 10 μl of NASBA buffer and pre-incubated at 65°C for 5 min before incubation at 41°C for 5 min.

The resulting complexes were incubated at room temperature for 10 min before incubation with cells.

To rule out nonspecific binding, frozen sections were incubated in 1 μM random sequences for 5 min. Then, SYL3C-Cy3 was diluted in 250 nM binding buffer and incubated with the sections for 20 min before incubation with DAPI for 3 min to clearly show the position of the nucleus.

After washing, cochleas were incubated in buffer (Vector Laboratories Inc. SK-41000) for 20 min before incubation in diaminobenzidine (Vector Laboratories Inc. SK-41000) for 6 h.

PC-3 cells were pre-incubated with RM2 (1000 nM in PBS, 37 °C for 5 min) before incubation with BODIPY-BBN (10 nM in PBS, 37 °C for 30 min).

Pulmonary arteries were incubated in the spin trap solution containing 500 µM 1­hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidin (CMH, Noxygen), 25 µM deferoxamine (Sigma) and 5 µM N,N-diethyldithiocarbamate (DETC, Sigma) in Krebs-HEPES at 37°C for 45 min. 5-HT 0.1 mM was added during the spin trap incubation; PEG-SOD 300 U/mL was incubated during 45 min before spin trap incubation.

The cells were washed in PBS (3×, 5 min each) before incubation with 100-µl of Alexa-488- and Alexa-568-conjugated anti-rabbit or anti-mouse immunoglobulin G (Molecular Probes) diluted (2 µg/ml) in PBS containing 3% BSA (60 min at room temperature in a light protected humidity chamber).

Unfixed frozen cross sections (5 µm) were incubated with dihydroethidium (DHE) (5 µM; Invitrogen, Darmstadt, Germany) in a light-protected moist chamber at 37°C for 30 min. Before incubation with DHE, serial sections were treated with either l-NAME (100 µM), pegylated SOD (PEG-SOD) (250 U·mL−1), oxypurinol (100 µM) or VAS3947 (10 µM) for 30 min at 37°C.

Lysates were cleared (14 000 g, 4 min, 4 °C) before incubation with EphA2 antibody (1.5 μg) for 2 h.

The next day, the sections were washed in PBS 3 times for 10 min each before incubation with goat anti-IgM Alexa Fluor 488 (1 700; Invitrogen) for 1 hr.