Sentence examples for min before transfer from inspiring English sources

After brewing was complete, the coffee was left in the collecting chamber of the pots for 5 min before transfer to 250-mL sample bottles for subsequent analysis.

The tissue was then washed twice in 0.1 M PBS (pH 7.2) and immersed in 50% ethanol for 10 min before transfer to saturated uranyl acetate in 70% ethanol for 16 hr at 4°C.

Cellular samples (100 µL) and equal volumes of FDG staining aliquots (1∶10 dilution of the 20 mM FDG stock solution) were preheated at 37°C for 10 min. FDG was loaded onto cells and the solution was incubated at 37°C for 1 min before transfer into 15 mL Falcon tubes containing 2 mL ice cold HBSS+ buffer.

Specimens were subjected to stringent wash at 65°C for 10 min before transfer to a CISH wash buffer.

The animals were allowed to recover on a wet tissue for 10 min before transfer to water.

Standard freezing conditions involved suspending cells in 5% DMSO in FCS at a density of 4 × 10 cells per ml and placing them on dry ice for 90 min before transfer to liquid nitrogen.

For the high magnification DIC images, 96-well µclear flat bottom plates (Greiner) were coated with 0.1 mg/ml poly-D-lysine (Sigma) for 5 min and allowed to air dry for 5 min before gently transferring 100 µl of culture to the well with a cut-off pipet tip.

For the confocal slices through rosette colonies, sterile, 8-well µ-slides (Ibidi, Germany) were coated with 0.1 mg/ml poly-D-lysine (Sigma) for 5 min and allowed to air dry for 5 min before gently transferring 250 µl of culture to the well with a cut-off pipet tip.

To facilitate DNA protein binding, we mixed the ds-oligo pool with 10 µg nuclear extract in 1× incubation buffer (Affymetrix, Santa Clara, CA, USA or Signosis, Santa Clara, CA, USA) at 15 oC for 30 min before transferred to the center of the Spin Columns.

Samples were then completely dried with N2 and baked at 100 °C for 3 min before transferred into the MOCVD reactor (Aixtron 200) for the GaAs NWs growth.

The mixture was stirred for 10 min before transferring to a 50-mL Teflon-lined stainless steel autoclave, which was later kept at 200 °C for 12 h.