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As part of a study on the physiological effects of lolitrem B and paxilline in mice we monitored cardiovascular function and observed unexpected responses to these compounds.
To further determine whether differences in physical activity contributed to the higher energy expenditure/metabolic rate of older Ghsr-/ mice, we monitored spontaneous locomotor activity of these mice while in the calorimetry chambers.
To verify that Bcl-x was efficiently deleted in the tumors arising in RIP1-Tag2; RIP-Cre; Bcl-xfl/fl mice, we monitored recombination using a PCR-based assay on genomic DNA from 12 randomly picked tumors (range 5.2 mm3–229 mm3): 10/12 tumors exhibited complete recombination, 1/12 showed partial recombination, and 1/12 had no recombination (Figure 4A and data not shown).
In some treated mice we monitored body temperature before and 30 45 min after vector or protein injection(s) and assigned an allergy score (Li et al, 2003; Sun et al, 2010).
To define the impact of the UPR on the cellular environment in the injury zone of atf4−/− mice, we monitored glial reactions 5 and 14 days after surgery, at the beginning of the locomotor recovery phase according to the BMS score.
To ensure that the presence of a tumor did not affect pregnancy, lactation, or involution in these mice, we monitored average litter size at the time of weaning and ensured they were comparable between mice that bore tumors at involution day 2 (n = 9) and mice that did not (n = 49).
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Of the nine OVαβ TCR mice that we monitored for the complete twelve months, only one mouse developed EAE.
To determine the onset of Peril −/− mice lethality, we monitored survival at both early and late stages of embryonic development.
To investigate whether accelerated atherosclerosis in DKO mice decreases longevity, we monitored DKO (n=20) and apoE−/− mice (n=20) on standard chow for 18 months.
Using a series of deletion constructs of the mouse hr gene, we monitored the sub-cellular localization of the recombinant protein by in situ immunolocalization and biochemical fractionation after nuclear matrix extraction of transiently transfected cells.
To visualize the clearance efficiencies of the peptide fragments, we monitored mice by in vivo fluorescence imaging and observed a strong increase in fluorescent signal that was localized to the bladder of thrombotic mice relative to controls.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com