While we did not find heroin dose-related changes in mRNA expression levels in C57 mice, we did observe dose-related differences in 129 mice.
In the present TLC analysis, although very little metabolism and degradation of [11C]AZT occurred in vivo in mice, we did observe three hydrophilic metabolites of [11C]AZT, two of which were observed in the bile (metabolite b and c, Fig. 6).
However, in the control mice, we did not find any PDX1+ proliferating cells (Fig. 3A).
In uninfected mice, we did not find differences in the B lymphocytes (Figure 2A and 2B, respectively).
In 13 week old Hexb−/−FcRγ+/+ and 15 week old Hexb−/−FcRγ−/− mice, we did not see this B cell expansion.
Because of the embryonic lethality of Smad5−/− mice, we did not further generate Smad cKO/cKO Smad5−/− mice so that we could study the postnatal phenotype in detail.
In hearts from fat-fed versus chow-fed human apoB-transgenic mice we did not identify this difference in GLUT4 and PFK mRNA expression (Fig. 4B).
In the current study in anesthetized mice, we did not see a difference in baseline heart-rate when comparing A1AR−/− mice to littermate controls.
In survival experiments using C3 deficient mice, we did not find a major role of the complement system for the survival after post-exposure immunization (Fig. 5C).
In contrast to the glucose intolerance we observed in male mTR−/−G4 mice, we did not identify baseline impaired glucose tolerance in female mTR−/−iG4.
While naïve wild-type (WT) mice had a higher percentage of CD44int/hi CD8+ T cells than uninfected IL-15 knock-out (KO) mice, we did not observe significant differences in CD44int/hi frequency between infected WT and KO mice.
