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Using knock-out mice we chose to study cataractogenesis in the lens and oncogenic transformation in mouse embryo fibroblasts to assay for effects of ATM deficiency.
To minimize manipulation and distress in the infected mice, we chose to use the quinolone enrofloxacin (15 mg/kg), which only required subcutaneous injection every 24 h.
Because we were unable to produce [APPswe/PS1dE9]/RAP mice, we chose to compare the parental lines of APPswe/PS1dE9 animals, which were wild-type with respect to RAP, to F1 and F2 [APPswe/PS1dE9]/RAP mice.
In order to reduce confounding factors in our experiments, such as undetected pathology in old mice, we chose 20 months as a maximum age.
To assess critical translational benefit of estrogen in male mice, we chose the timing of injection at 15 min after ROSC and evaluated three doses.
Because LDPI scans are disrupted by motion [ 44] and single or combined sedatives have lacked restraining effects in mice, we chose to perform our study under 1.5% isoflurane anesthesia to record reference perfusion values.
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To address these questions in a mouse model we chose APP23 mice, which overexpress human APP with the Swedish mutation (KM670/671NL) driven by a Thy1 promoter [ 21].
Because previous work had revealed that Piry virus neuroinvasion targets hippocampal fields [19] and that Wisteria floribunda histochemical staining conspicuously defines the architectonic limits of the hippocampal fields in adult mice [23], we chose to limit the quantitative neuropathological analysis to CA3.
Of these viruses (isolated in 2005), 2 had a substitution at residue 627 in PB2 that is associated with pathogenicity in mice (33 ); we chose A/chicken/Krasnodar/123/2006 because it was the only virus isolated in 2006.
To study type 2 diabetes in a mouse model we chose the leptin receptor knock out mouse model (Leprdb).
As the network was largely constructed from knowledge of human and mouse physiology, we chose to retain for this protein both its rod- and cone-specific interactions.
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