To gain insight into the functions of α-pv in ECs, we intercrossed mice carrying a loxP-flanked α-pv gene (α-pvfl/fl) with mice expressing the Cre recombinase under the control of the Tie2 promoter (Tie2-Cre).
In order to study the role of ADF and CFL1, the two main actin-severing forms of cofilin in epithelial cells (Vartiainen et al., 2002), we intercrossed K14CreERT2 mice with ADF−/− mice and with mice expressing CFL1 flanked with loxP sites (CFL1 fl/fl ) or with mice that were both ADF−/− and CFL1 fl/fl.
For this, we intercrossed mice carrying the Apc 1322T allele (Pollard et al., 2009) to Lgr5 GFP-CREER mice.
By intercrossing the mice, we obtained homozygotes of the MSM genotype for the loci of interest, and established a series of congenic strains.
However, on intercrossing Tg Pkd1l2)/0 mice, we found that homozygous Tg Pkd1l2)/ Tg Pkd1l2) mice showed extreme growth retardation and developed progressive hind limb dragging and paralysis between 4 and 6 weeks of age.
Then, we intercrossed mice heterozygous (p8+/-) for the targeted disruption to produce homozygous offspring (p8-/).
To do so, we intercrossed mice heterozygous for a Blimp1 mutant allele (Ohinata et al., 2005) and retrieved 8-cell stage embryos.
We intercrossed mice heterozygous for a (7.18) Robertsonian translocation (14) and have genotyped 1,812 embryos from 364 litters with known parental origin, strain and age (15).
To test the hypothesis that a compensatory increase in EHD4 expression prevents the appearance of glomerular pathology in Ehd3 / mice, we intercrossed Ehd3 /– mice with the previously described Ehd4 /– mice [28], [31] to generate Ehd3 /–; Ehd4–/– double-null mice.
To prove the ΔCS mutation accounted for the accelerated loss of RGCs via the Fas receptor, we intercrossed ΔCS mice with Fas-deficient lpr mice.
