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Exact(6)
To evaluate the impact of these mutations on H. pylori viability, these strains were transformed with the suicide plasmid pILL796 designed to delete the chromosomal copy of ssrA and the number of transformants on selective medium were counted (Fig. 1).
The detached cells collected from rinsing solution and medium were counted using light microscopy.
After incubation, the various colony types on each medium were counted.
18 Bacteria in basolateral medium were counted by plating appropriate dilutions at 1, 3, 6 and 8 h after inoculation.
After 24 h (t24h) non-adhesive cells in the medium were counted in every well and the adhesive cells (N0) were calculated.
The interlayer of PBMCs consisting of lymphocytes and monocytes was separated and diluted with 10 ml of culture medium, centrifuged for 5 min at 200 × g and the washed cells suspended in fresh culture medium were counted in a Burker camera.
Similar(54)
The medium was replaced with fresh medium daily for 7 days, and radioactivity in the replaced medium was counted.
The number of colonies on each medium was counted after 5 days of incubation at 30°C.
The cell number per ml of medium was counted using a hemacytometer and recorded every day until the eighth day.
The radioactivity released into the medium was counted from 100 μl portions of the supernatants.
After trypsinization and resuspension in complete medium, cells were counted with a Coulter Z2 instrument, 1.0 × 106 cells from each sample were pelleted by centrifugation, washed by gentle resuspension in Dulbecco's phosphate-buffered saline, centrifuged again, and suspended in staining buffer.
Related(20)
medium were conducted
medium were represented
medium were computed
media were counted
medium were studied
medium were sterilized
medium were included
medium were poured
medium were extracted
medium were removed
medium were served
medium were recorded
medium was counted
medium were used
medium were injected
medium were preincubated
medium were introduced
medium were added
medium were sent
medium were measured
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