For longer strands, droplets of cell medium were added to prevent dehydration as bioprinting of longer strands took longer time.
Hydrodynamic and light attenuation through the medium were added in the model to obtain a realistic representation of photobioreactor.
Varying concentrations of the chemical compound in cell culture medium were added into each well (the final concentrations were 187.5 nM 1.5 µM) in quadruplicate.
Different concentrations from the tested sample in fresh medium were added after 24 h of seeding.
After 30 min, 200 μL co-culture medium were added to each well.
Alkali, acid and feed medium were added into the culture using peristaltic pumps.
To this medium were added varying concentrations of human plasma-derived AB-PDGF and alpha 2 M, alone or in combination.
Test extract was diluted appropriately in DMEM medium, were added to the cells and again incubated for 48 h.
B16F10 cells at a density of 2 × 104 cells/ml in complete medium were added to 40-mm tissue culture plates and incubated for 48 h.
After 30 min, 1200 cells for SW-13 in 20 µl of medium were added per well and then cultured at 37 °C.
After culturing for 1, 3, and 5 days, 0.1 mL of MTT solution and 0.9 mL of medium were added to each well.
