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Thereafter, the discrete colonies formed on growth media were counted and recorded as colony forming units per gram (CFU g−1) for bacteria and spore-forming unit per gram (SFU g−1) for fungi.
HepG2 cells suspended in antibiotic free media were counted and plated into the 6-well plates at the same cell number per well.
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All arterial rings used for the organ culture were fixed and double-stained with anti ?-actin antibody and DAPI, and the total cell number in the whole cross-sectional area of the tunica media was counted (Figure?2C).
After 3 days at 30°C, the number of colonies capable of growing on glycerol media was counted.
Following this procedure, the number of BrdU-labeled positive cells in the intima and media was counted [ 23].
A Sky News spokeswoman, aware that the world's news media are counting on its coverage, said she anticipates that the crew will have "enough time to get airborne".
Radioactivities present in supernatants, lavage media, and cells were counted to estimate the percentage of cell-associated I-labeled IGF-1.
Mosquito midguts were dissected, fixed for 30 min at room temperature in 4% paraformaldehyde in PBS, mounted in slides with Vectashield mounting media and oocysts were counted under an ultraviolet microscope.
At the appropriate time point, 24, 48 and 72 hours post-transfection, cells were trypsinised and re-suspended in 1 ml of media and nuclei were counted in triplicate for each sample using a Beckman Coulter Cell counter (Beckman Coulter Inc, Brea, CA).
Flies were reared at a maximum of 20 per tube and transferred every other day into fresh media and dead flies were counted.
Then, the apoptotic cells in the tunica media of each experimental condition were counted, respectively.
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