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Only FBS based freezing medium were used in (A D).
Cells incubated in exosome-free medium were used as a control.
Cells incubated in exosome-depleted medium were used as a control.
Solvent control-treated cells (0.025% DMSO in medium) were used as control for etoposide.
For RNA FISH, THP-1 cells, or CD34+ PB HSPCs differentiated for 11 days in granulocytic differentiation medium were used.
Suspensions of harebell cells in culture medium were used as an in vitro model system.
BMGY, custom-made methanol medium and β-estradiol-containing salt medium were used in the experiment for initial outgrowth, IFNα2b production and rHGH production, respectively.
Samples administered EtNBS at known concentrations (250 nM and 500 nM) in complete culture medium were used as references for comparison.
Different media formulations namely BMGY, yeast extract peptone dextrose (YPD), BMMY, custom-made methanol medium and β-estradiol-containing salt medium were used in these experiments.
There was no inoculation and only microorganisms indigenous to the bed medium were used throughout the whole process.
For this purpose, sodium borohydride (NaBH4) solutions ranging between 0.01 and 0.06 M in alkaline medium were used.
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