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The medium was replaced with fresh medium daily for 7 days, and radioactivity in the replaced medium was counted.
The cell number per ml of medium was counted using a hemacytometer and recorded every day until the eighth day.
The number of colonies on each medium was counted after 5 days of incubation at 30°C.
The radioactivity released into the medium was counted from 100 μl portions of the supernatants.
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To evaluate the impact of these mutations on H. pylori viability, these strains were transformed with the suicide plasmid pILL796 designed to delete the chromosomal copy of ssrA and the number of transformants on selective medium were counted (Fig. 1).
The detached cells collected from rinsing solution and medium were counted using light microscopy.
After incubation, the various colony types on each medium were counted.
18 Bacteria in basolateral medium were counted by plating appropriate dilutions at 1, 3, 6 and 8 h after inoculation.
After 24 h (t24h) non-adhesive cells in the medium were counted in every well and the adhesive cells (N0) were calculated.
The interlayer of PBMCs consisting of lymphocytes and monocytes was separated and diluted with 10 ml of culture medium, centrifuged for 5 min at 200 × g and the washed cells suspended in fresh culture medium were counted in a Burker camera.
Radioactivity present in medium, lavage medium, and explants was counted.
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