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The medium were poured into layer of a 5 mm deep.
Therefore, few drops of medium were poured into each well before inserting a ChM piece in a well.
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(1 Between 40 and 45 °C, the medium was poured into petri dishes.
At 45 °C, about 20 mL of each medium was poured into a sterile petri dish.
Next , 2750µL of the enriched broth nutrient culture medium was poured into a test tube.
The medium was poured into sterile Petri plates and cooled at room temperature.
Next day, a second layer of the same medium was poured on the top.
In case of the assessment for cultivated cells, the same volume of culture medium was poured in cell culture dish covered with the cells at confluence.
In brief, 200 µl volume of nutrient medium was poured in each well and inoculated with 20 µl inocula of 48 h grown cultures.
After incubation at 26 °C in the dark for 1 day, 10 mL of fresh hygromycin B-containing medium was poured on the surface of the original one.
A 20-mL volume of the medium was poured into a Petri dish (diameter, 90 mm) on a horizontally leveled surface.
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