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Exact(43)
Thirty-five microfiters of medium were removed from each well, and 30 μl CellTiter‐Glo cell viability reagent was added.
Then, the medium were removed and replaced with 100 μL fresh culture medium.
Finally, the medium were removed and 150 mL dimethyl sulfoxide (DMSO) was added into each well.
To assess the cellulolytic potential of the fungi when grown on microcrystalline cellulose samples of culture medium were removed at various times and assayed for endoglucanase activity.
Samples of the release medium were removed at designated times using a sample probe with an inline 0.45-μm filter to prevent removal of the microparticles during sampling.
At fixed time intervals (0, 0.5, 1, 2, 4, 6, 8, 24, 48, 72, and 120 h), 5 ml portions of the medium were removed, and fresh medium was added to maintain sink condition.
Similar(17)
After treatments, culture medium was removed and washed with PBS.
Then the medium was removed and the substrates were rinsed with PBS three times.
One hour after transfection, the medium was removed and cells were washed with PBS.
Then the medium was removed carefully, and 100 μl DMSO was added to dissolve formazan crystals.
The inorganic carbon of the fresh medium was removed by acidification and stripping with N2.
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