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Exact(5)
Using response surface methodology (RS M, a second-order polynomial equation was obtained by multiple regression analysis and a yield of 6.37 g/L of PHB dry weight was achieved from the optimized medium at pH 9. The same medium was utilized for fermentor studies by fed-batch culture.
A total digestion of proteins, present in the growth medium, was utilized using Pronase (Boehringer Mannheim) at a concentration of 2 mg/mL in each sample.
Ammonium sulfate in the batch medium was utilized in the first 40 hours and the amount of ammonium hydroxide added to correct the pH thereafter did not result in ammonia accumulation until 95 hours (Figure 3b) while supporting maximum cell density of 235 OD600 (Figure 3a).
In our study, incubated medium was utilized to dilute the extracted solution and to be the control sample.
The lipid content of Scenedesmus obliquus reached up to ~58.3% (dry cell weight) when lower nitrogen-supplemented medium was utilized [ 10– 13].
Similar(55)
Commonly, the well-established Cooper medium, a glucose-based mineral salt medium, is utilized for the microbial production of Surfactin.
In this method, the temperature difference potential between LNG and cooling medium is utilized step by step.
A total of 8 nalgene bottles with 250 mL liquid medium were utilized, 4 for each of the fungi, control (monospecific), mixed (including both species), FhrAPS (each of the fungi specimens cultured in the presence of the recombinant protein), and mixed-FhrAPS (with the two fungal species).
The changes before and after treatment with Neochloris sp. were measured; among four heavy metals that were presented in effluent medium only lead (Pb) was utilized by the strain.
Additionally, medium supplemented with caspofungin acetate was utilized to screen for genes which impact 1,3-beta-glucan synthase activity and/or antifungal resistance.
Therefore, a richer medium, BA medium supplemented with 0.2% yeast extract was utilized.
Related(20)
language was utilized
vector was utilized
instruction was utilized
media was utilized
medium was refreshed
medium was provided
medium was selected
medium was renewed
medium was replaced
medium was purchased
medium was collected
medium was changed
medium was discarded
medium was disposed
medium were utilized
medium was harvested
medium was named
medium was exchanged
medium was maintained
medium was performed
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