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Supernatant medium was collected by centrifuge.
Four weeks later the developed purple medium was collected.
The conditioned medium was collected after another 48 h culture.
The cell medium was collected and cell count was done with a hemocytometer.
At established time points, medium was collected and analysed for Ca2+ in solution and pH.
Conditioned medium was collected and centrifuged at 3000g for 10 min at 4 °C.
Conditioned medium was collected for 7 days.
Conditioned medium was collected after 2 DIV.
Infectious supernatant medium was collected on day 5 pi.
The medium was changed and the conditioned medium was collected.
The medium was collected and cleared of non adherent cells.
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