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A total of 8 nalgene bottles with 250 mL liquid medium were utilized, 4 for each of the fungi, control (monospecific), mixed (including both species), FhrAPS (each of the fungi specimens cultured in the presence of the recombinant protein), and mixed-FhrAPS (with the two fungal species).
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Through its widespread efforts, CCNMTL is helping to bring quality content to the Internet and is also influencing the way the new medium is utilized.
In this method, the temperature difference potential between LNG and cooling medium is utilized step by step.
Using response surface methodology (RS M, a second-order polynomial equation was obtained by multiple regression analysis and a yield of 6.37 g/L of PHB dry weight was achieved from the optimized medium at pH 9. The same medium was utilized for fermentor studies by fed-batch culture.
Commonly, the well-established Cooper medium, a glucose-based mineral salt medium, is utilized for the microbial production of Surfactin.
A total digestion of proteins, present in the growth medium, was utilized using Pronase (Boehringer Mannheim) at a concentration of 2 mg/mL in each sample.
Ammonium sulfate in the batch medium was utilized in the first 40 hours and the amount of ammonium hydroxide added to correct the pH thereafter did not result in ammonia accumulation until 95 hours (Figure 3b) while supporting maximum cell density of 235 OD600 (Figure 3a).
In our study, incubated medium was utilized to dilute the extracted solution and to be the control sample.
The lipid content of Scenedesmus obliquus reached up to ~58.3% (dry cell weight) when lower nitrogen-supplemented medium was utilized [ 10– 13].
YEPD (2% (w/v) yeast extract, 2% (w/v) glucose and 0.1% (w/v) peptone) medium and CM (complete medium) [ 29] were utilized to repress the P alcA completely and partially, respectively.
Ionic liquids 1-butyl-3-methyl-imidazolium tetrafluoroborate ([BMIM]BF4), 1-butyl-3-methylimidazoliumhexafluorophosphate ([BMIM]PF6) and conventional medium tetrabutylammonium perchlorate (TBAP) were utilized as electrolyte for the preparation of poly(4,4′,4′'-tris[4- 2-bithienyl phenyl]amine) (PTBTPA) films and assembling of EC devices.
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