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In all culture conditions, the medium was renewed every day.
In all culture conditions, the medium was renewed every 48 h.
The medium was renewed with the same concentration of modified Steinberg medium every two weeks.
After this time, the assay medium was renewed, and the cells were incubated for another 24 h.
The culture medium was renewed every 2 3 days as well as before the application of the radioactivity.
Samples were immersed in 0.5 ml of the corresponding medium and this medium was renewed at every measurement.
This medium was renewed every two days.
Half of the culture medium was renewed each week.
The culture medium was renewed every other day.
Culture medium was renewed every 3 days after fibroblast plating.
The medium was renewed and cells were cultured for another 2 h period prior transfection.
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