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The characterization of the medium was performed using standard instrumental analysis.
Time dependent numerical simulation of relativistic wind interaction with interstellar medium was performed.
The identification of possible limiting nutrients in the culture medium was performed by the biomass elemental composition approach.
DHM of HCT-8 cells exposed to LB226692 OMVs (4.7 μg/ml of protein containing 580 ng/ml of Stx2a) or culture medium was performed using an incubator-integrated microscope (iMIC, Till Photonics, Gräfelfing, Germany) with a DHM module (engineered at the Center for Biomedical Optics and Photonics, University of Muenster, Germany)57,58.
The electroreduction of nitrogen species in alkaline medium was performed on platinum nanoparticles (1 2 nm) with metal loadings of 5 and 10 wt% Pt, synthesized by the chemical carbonyl route.
The impact of PtNPs on bacterial growth in liquid medium was performed as follows.
The pH control of the medium was performed using a pH meter I-160MI.
A serial transfer of 50 mg of cells (dry weight) into fresh medium was performed every 24 h.
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Benchtop bioreactor tests with the optimized medium were performed.
Repeated cycles of IgG purification from a cell culture medium were performed, each followed by cleaning with aqueous NaOH (0.1 M, 0.5 M and 1 M).
The stability study of graphene oxide and reduced graphene oxide (rGO) in aqueous medium were performed by visual observation and surface charge measurement.
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