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GFP-tagged LC3 expression vector was utilized to demonstrate the occurrence of autophagy.
A TMV-based ICON expression vector was utilized for efficient expression of NaVCP.
The pGL3 empty vector was utilized to keep the amount of DNA transfected constant.
In contrast to PdG, the replication fidelity of the γ-OH-PdG adduct 2 varied in mammalian cells depending on whether a single- or double-stranded vector was utilized (Table 1, rows 11−14).
The reduced acrolein-mediated N-dG: N-dG interstrand cross-link was site specifically incorporated into a DNA vector, and this modified vector was utilized to transform wild-type E. coli strain and pol II and pol IV deletion mutants (147).
We generated a panel of 786-O cells as previously, with the following exceptions: 1) VHL and control expression constructs were transfected rather than selected into these cells; 2) pVHL19 expression constructs were used (rather than pVHL30); and 3) a different expression vector was utilized (see Methods).
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A power quality level index vector is utilized in the proposed infrastructure.
In this algorithm, a time-varying base vector is utilized in conjunction with the quasi-equivalent aggregation strategy.
First, the Newton center interpolation formula and the linear interpolation formula, together with a modified rectangular rule and an augmented state vector, are utilized to construct the equivalent digital linear models.
Interval analysis and a double-deck gradient-based method with GA estimating the initial range of drift vector are utilized to obtain the sufficiently small intervals including the global minimum velocity increment and the global minimum solution or one sufficiently close to it in these intervals.
Moreover, the BITCEM motion vector is utilized as the initial search point for near-still or slow BITCEM motion types.
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