Sentence examples for medium was discarded from inspiring English sources

Before the assay, culture medium was discarded.

Following incubation, medium was discarded and cells were washed three times in PBS.

After 72 h, cell culture medium was discarded and the cells were washed twice with PBS.

The medium was discarded and 50 μL 1× lysis buffer was added to each well.

Culture medium was discarded and was replaced with 150 μL DMSO.

The medium was discarded and replaced with DOX solution and DOX nanoparticles.

The medium was discarded, 150 μl of DMSO was added to each well, and plates were incubated for 10 min.

At the designated time points, the medium was discarded and the cells were rinsed twice with cold PBS.

Next, the culture medium was discarded, and the cells were trypsinized, centrifuged, and washed three times with PBS buffer.

Subsequently, the cell medium was discarded, and the cells were lysed with 0.004 N HCl in isopropanol.

The medium was discarded and the formazan blue crystals formed were dissolved with 50 μl of DMSO.