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Exact(19)
About 400 ml of culture liquid medium containing appropriate Rhizobium sp. were added to 1 kg of the carrier (sterile fine filter mud) and mixed thoroughly and then packed in plastic bags.
The cells were incubated for 24, 72, 120 and 168 h (for 120- and 168-hr incubations, the medium was replaced at 72 h with fresh medium containing appropriate concentrations of MNPs).
These results indicate that the culture medium containing appropriate quantity of Glycine and Triton X-100 is favourable to the secretion and expression level of ZZ EGFP in gene-engineering bacteria Escherichia coli HB101.
Transformants were grown at 37°C and 200 rpm in LB medium containing appropriate antibiotics to an OD600 of 0.4 to 0.6.
Expression of recombinant proteins (His8-tagged full-length human metavinculin, metavinculin-ΔLeu954, VH, Vh2 Vh3, MVt, MVt-ΔLeu954, MVt-R975W, Vt, GST-Vt, and GST-ΔN-Vt) was performed by growing transformed BL21-DE3 cells at 37°C in Luria-Bertani medium containing appropriate antibiotics.
Cells were grown in LB- or minimal medium containing appropriate carbon sources and antibiotics.
Similar(41)
Samples were mixed with an equal volume of medium containing appropriated amounts (see figures notes) of rhu IFN-γ (Heber Biotec, Havana; 1.0 × 107 IU/mg).
The culturing medium contains appropriate amounts of K2HPO4, (NH4 2SO4, MgSO4·7H2O, Na-succinate, yeast extract and EDTA FeCl3 complex.
The oligosaccharide medium contained appropriate amounts of oligosaccharides solution and was supplemented with 0.1 g/L (NH4 2SO4, 1.0 g/L yeast extract, 2.7 g/L KH2PO4, 2.4 g/L Na2HPO4 · 12H2O, 0.2 g/L MgSO4 · 7H2O, 0.1 g/L EDTA disodium salt and 1% (v/v) trace element solution, pH 5.5.
Luria-Bertani medium (5 mL) containing appropriate antibiotics was inoculated with three individual colonies and cultured overnight at 37°C, 220 rpm.
Terrific Broth medium (50 mL) containing appropriate antibiotics was then inoculated with 0.5 mL of the overnight culture and grown in a 250 mL baffle flask at 37°C and 300 rpm until an optical density at 600 nm of at least 0.8 was reached.
Related(13)
medium containing corresponding
vector containing appropriate
media containing appropriate
medium containing delipidated
medium containing individual
medium containing such
medium containing economic
medium containing heavy
medium containing extracellular
medium containing fresh
medium containing nocodazole
medium containing vitamin
medium containing ammonium
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