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The recombinants were cultured in media containing appropriate antibiotics, ampicillin (100 μg/mL) and erythromycin (200 μg/mL for E. coli and 5 μg/mL for L. reuteri).
For chemical treatment, the cells were cultured in serum-free media containing appropriate concentrations of EtOH or ACR.
Media containing appropriate treatments were added and tissues incubated for a further 24 h prior to formalin fixation/paraffin embedding.
Cells were maintained in selective media containing appropriate concentration of G418 for 13 days, until the control (untransduced) MSC were dead.
All non-combination wells were washed at 20 h and media containing appropriate agents replaced, to control for media change and fresh agents in the combination group.
For longer incubation periods (i.e., days 4 and 5) culture media were replaced after 72 h with fresh media containing appropriate ECGC or EA concentrations.
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For maintenance of resistance plasmids, media contained appropriate concentration of antibiotics.
Cells containing GAL1 promoter regulated CPY* and its variants were grown at 30°C in synthetic media containing the appropriate amino acids and 3% raffinose to mid logarithmic phase.
Cells were maintained at 37°C using a Bioptechs Delta Controller and fresh media containing the appropriate ligand was cycled over the cells.
Auto-induction medium was inoculated with overnight cultures of LB media containing the appropriate construct.
Strains were grown in complete (CMA) or minimal (MMA) media containing the appropriate supplements (Cove 1966).
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