Briefly, osteoblasts were cultured in medium containing vitamin C (50 μg/mL) and β-glycerophosphate (10 mM) for 2 wks, and the medium was changed every 3 d.
To investigate whether NCFM degraded vitamin E acetate, we performed an in vitro digestion experiment where NCFM was grown 24 h in medium containing vitamin E acetate.
However, to be cultured axenically, Dehalococcoides requires a specialized reduced medium containing vitamin B12 (Löffler et al., 2012); despite significant efforts, Dehalococcoides grows much more slowly and to lower cell density in axenic culture compared with mixed cultures.
A volume of 50 μL of ABTS or DPPH was added to each well at final concentrations of 50 and 20 μM respectively after incubation for 1 h in medium containing vitamin C or Spirulina extract at 25, 125 and 250 μg/mL.
Alizarin red-S staining showed that mineralized nodules formed when osteoblasts were cultured for 2 wks in medium containing vitamin C (50 μg/mL) and β-glycerophosphate (10 mM), and this increased in a concentration-dependent manner with the addition of SWT.
Ten IZEs were inoculated on each Petri dish (90 mm) containing approximately 20 ml of autoclaved basal Gamborg's B5 medium (containing vitamins; Duchefa Biochemie, The Netherlands) supplemented with 20.0 g/L sucrose (BDH Laboratory supplies, UK), 3.5 g/L Phytagel (Sigma-Aldrich, USA), 5.0 μM of 2, 4-D (Sigma-Aldrich, USA) at pH 5.8 ± 0.01.
Seedlings of Arabidopsis thaliana (Columbia 0 ecotype) were grown under sterile conditions on a half strength Murashige and Skoog (MS) medium containing vitamins (Duchefa, Haarlem, Netherlands), supplemented with 10 g/L sucrose and solidified with 4 g/L Gelrite (Duchefa) at pH 5.7.
Other foods containing vitamin D are listed here.
Use antioxidant cream (containing vitamin C).
MEFs were infected twice, 2 days post virus infection, and MEFs were supplemented with KSR medium containing 10 ng/ml vitamin C, 1/100 N2 supplement and 10 ng/ml bFGF.
rFVII was produced from cell lines growing adherently in roller bottles or triple-T-flasks (both from Nunc, Thermo Fisher Scientific, Waltham, MA, USA) after withdrawing FBS, and, after washing cells once with phosphate-buffered saline without Ca and Mg (Life Technologies), in medium containing 10 μg/mL vitamin K1 (Sigma, St . Louis MO, USA) for 24 h.
