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Consistent with the accumulation of TPX2 during meiotic maturation, we observed a progressive increase in the signal to noise ratio of the TPX2 labelling (Fig 1D).
However, the magnitude of T cell proliferation induced by the four viruses correlated well with the extent of MDDC maturation we observed previously [30], as well as in additional MDDC maturation studies (data not shown) in which IAV was slightly but not significantly stronger in inducing MDDC maturation than rHPIV3, rHMPV, and rHRSV.
In addition to synaptic maturation, we observed a significant synaptic elimination in adult WT animals illustrated by the lower frequency of miniature EPSCs recorded in LA principal cells in presence of the voltage-sensitive sodium channel blocker Tetrodotoxin (TTX, 10 µM) in adult animals (Table S1).
Regarding T-cell maturation, we observed a predominance of T-IV subtype (38.0%).
The reduced dendritic branching and delayed maturation we observed might be due to deficits in the neuronal transport mechanism or abnormalities in cytoskeleton structures.
Besides identification of genes and pathways known to be involved in DC differentiation and maturation, we observed dynamic DNA methylation changes at many novel genes, most of which are demethylated.
Similar(50)
To investigate whether the ventricular maturation abnormalities we observed had long-term consequences, we allowed Scrib f/f Nkx2.5-Cre litters to be born.
This different dosage possibly explains the maturation effects we observed, which are, however, demonstrated by changes relative to many differentiating markers.
While this level of hypoxia did not directly lead to MoDC maturation we did observe a synergistic effect on LPS induced phenotypic maturation of MoDC in the presence of hypoxia.
However, in 1% O2 we observed maturation of OLs (Figs. 8 and 9).
Following infection of MyD88KO mice, we observed reduced maturation of both macrophages and DCs compared to WT mice again suggesting a role for the MyD88 pathway in the maturation of APCs (Figure S1C,D).
Related(20)
development we observed
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