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To understand how HCV infection interferes with TLR4-induced maturation, we analyzed the phenotype and the cytokine secretion of mature cells.
To identify genes and pathways associated with pulmonary maturation, we analyzed RNA-seq experiments from mouse whole lung samples collected at seven developmental time points, embryonic day 16.5 (E18.5), E18.5, postnatal days 1, 3, 7, 14, and 28 (Fig. 5a; Supplementary Table 2).
To quantify the changes in Syp-GFP subcellular distribution during GC maturation, we analyzed the percentage of Syp-GFP localized to the axonal region versus the dendritic region for mature and immature GCs.
To test whether Dicer plays a role in pre-rRNA maturation we analyzed pre-rRNA processing in ES cells by pulse labeling the RNA with [3H-methyl]methionine for 30 min and chasing the label with nonradioactive methionine for 30 and 60 min. Incorporation of the label into pre-rRNA in Dicer−/− cells was about two times lower than in control Dicer+/− cells (Fig. 5A).
To monitor the effect of CPTH6 on cargo incorporation, which occurs concurrent with autophagosome maturation, we analyzed colocalization of LC3B protein with the adaptor protein p62.
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To evaluate the influence of unfavorable intrauterine conditions and catch-up growth, we analyzed growth and pubertal maturation in monozygotic twins with significant intra-twin birthweight differences.
To better understand the influence of EE on RGC circuitry development, we analyzed the maturation of RGC dendritic stratification in EE and non-EE mice.
Expression of a dominant-negative form of Mad2 interferes with the SAC in metaphase I, and a knock-down approach using RNA interference accelerates anaphase onset in meiosis I. To prove unambigiously the importance of SAC control for mammalian female meiosis I we analyzed oocyte maturation in Mad2 heterozygote mice, and in oocytes overexpressing a GFP-tagged version of Mad2.
After 4 hours of incubation no significant changes in maximal oxygen consumption (state 3) was observed (Fig. 12A) Since LPS can induce generation of ROS in human primary MoDC, which in turn might play a role in the maturation process we analyzed the generation of ROS using flow cytometry.
In this study, 14,592 (75%) of the expressed genes are represented in all three stages of embryo maturation that we analyzed and 12% are expressed in a single stage.
In order to study complete maturation of Mks we analyzed proplatelet formation (PPF) and we found that Mks, differentiated in adhesion to hMSCs, extended a significantly higher number of proplatelets when compared to control (Figure 3D).
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