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To confirm the positive regulatory role of ectopically expressed QKI-6 and QKI-7 in Schwann cell maturation, we used a knockdown approach using RNA interference to reduce the expression of the endogenous QKI-6 and QKI-7 isoforms.
To probe Dp71 expression in synapses of pyramidal cells and examine its putative role in synapse organization and maturation, we used immunofluorescence in primary cultured cells from mouse cortex and hippocampus.
To determine the possible involvement of HMGB1 in NK-dependent DC maturation, we used glycyrrhizin, known to interact specifically with soluble HMGB1 molecule [32], and anti-HMGB1 antibodies (Fig. 3a).
For observation of later stages of maturation, we used Nikon SMZ 1500 stereomicroscope.
To determine the molecular mechanisms whereby HDIs accelerate osteoblast maturation, we used microarray analysis to compare gene expression changes in MC3T3-E1 cells treated with HDIs or its vehicle, dimethylsulfoxide (DMSO).
In order to determine whether CDK5 contributes directly to alterations in neuronal maturation, we used a pharmacological inhibitor of CDK5 (Roscovitine), or an siRNA-based approach, to inhibit CDK5 activity or expression, respectively.
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In the speed of growth and maturation experiment we used chips (area ≈ 11 cm) from soft alder (Alnus glutinosa) shade leaves that had fallen in autumn.
To evaluate the effect of platelet inhibition on fistula thrombosis and maturation failure, we used an antiplatelet agent, sarpogrelate hydrochloride, in our study.
To predict optimal maturation thresholds, we used the integral projection model for the life history of hatchery-reared chum salmon (Appendix A).
In order to identify the molecules involved in aNK-dependent maturation of iDC, we used multianalyte profiling (MAP) to map the key cytokines produced in 24 h culture of iDC, NK cells and aNK∶iDC.
To study dendritic maturation of newborn neurons we used doublecortin (DCX) immunohistochemistry.
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