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In order to determine the effect of DHA on phagosomal maturation, we assessed the incorporation of the acidotropic probe lysotracker into mycobacterial phagosomes.
To test whether culture supplementation to promote nuclear maturation, we assessed the polar body extrusion after culture of immature GV and MI oocytes in three different maturation conditions: 1) Commercial human oocyte maturation medium (Sage), 2) IVM-medium, and 3) IVM-medium supplemented with selected ovarian factors as described above and in Materials and Methods.
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To control for this effect, we assessed erythroid maturation in cultures depleted of macrophages both with and without hemozoin.
For this reason, we assessed particle maturation and release in freshly-infected T cells from hCyclin T1 transgenic mice.
To address the activation and the maturation status of circulating DC, we assessed the expression of CD86, CD62L and CCR7 on blood DC.
First, we assessed the maturation and levels of the major lysosomal enzyme cathepsin D. Cathepsin D is transported from the Golgi through late endosomes to lysosomes.
Due to the differential effects of inflammatory mediators on DC apoptosis, we assessed the functional maturation induced on Lin-HLA-DR+ cells following stimulation with a CC, poly I C or CD40L.
Aiming to identify factors that could improve the function of DC from breast cancer patients in a clinical setting, we assessed the functional maturation induced by three different types of stimuli currently under investigation for immunotherapy.
After showing the key role of JAK in TNF-induced caspase-1 expression and activity, we assessed its function on maturation of IL-18.
Since we have recently demonstrated that LF blocks NF-κB translocation [ 9], and we have shown here that LF treatment inhibits DC maturation, we sought to assess whether LF-treated DC could induce tolerance to a nominal antigen such as KLH.
Here, we assess the maturation patterns of coastal Atlantic cod (Gadus morhua) in Skagerrak, where studies using neutral markers have revealed genetically differentiated populations of this harvested fish within tens of kilometres of coastline.
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