Dicer-1 is required to cleave endogenously encoded microRNA (miRNA) precursors into mature miRNAs that regulate endogenous gene expression.
Results obtained from modulating endogenous gene expression using such engineered transcription factors are reviewed in depth.
Expression of genes encoding such designed transcriptional regulators enable the selective modulation of endogenous gene expression.
Microarray analysis was used to determine endogenous gene expression profiles modulated during nonviral gene transfer.
The leader sequence of the endogenous gene serves as the antisense target in the gene-switching method.
One goal of gene transfer may be to express a functional gene when the endogenous gene is inactive.
In hepatoma tissue culture cells, we measured trans-activation of the endogenous gene encoding tyrosine aminotransferase, a gluconeogenic enzyme.
Here we applied an inverse metabolic engineering approach to identify endogenous gene targets conferring improved alcohol tolerance in S. cerevisiae.
Following endolysosomal escape the AuNPs-PEI-FA.siRNA formulation produced enhanced endogenous gene silencing compared to the non-targeted formulation.
In this report, we describe the first application of this technology to regulate an endogenous gene in mammalian cells.
However, it is still largely unknown how small molecules activate the endogenous gene regulation network, and gradually enable cell fate transitions.
