Dynamics of heat shock factor association with native gene loci in living cells.
We show that inducible alkaline phosphatase activity correlates directly with native gene expression.
Next, we demonstrated the utility of finely tuning native gene expression by downregulating the abundance of phycobillisomes.
BAC transgenes usually retain the native gene architecture including all cis-regulatory elements as well as the exon intron configurations.
Extracts prepared from yeast cells expressing epitope tagged proteins under the control of native gene promoters were used for immunoprecipitations and Western blot analyses as described28.
We use this strategy to interrogate a library of transposon mutants and elucidate the native gene network influencing both cell fitness and thiamine production.
We found no evidence that these abnormalities were caused by interference of the construct with the function of the native gene, as judged by analysis of the site of integration of the promoter-GUS cassette, expression of the native gene and lolitrem B and ergovaline levels in infected plants.
Slider turtles, the southern species, are very closely related to painted turtles, the northern species; courtship and mating rituals are very similar, and when thrown together, interbreeding can occur, diluting the native gene pool.
Puromycin N-acetyltransferase expression was driven by the native gene regulatory elements of Chx10 in the transgenic ESC line, resulting in positive selection of V2a INs after induction and treatment with puromycin.
Introducing transgenes to minichromosomes does not have the risk of insertion within a native gene; additionally, transgenes on minichromosomes can be transferred between lines without the movement of linked genes.
As a follow-on to the reporter gene analysis, we report here the evaluation of genome-wide patterns of native gene expression within Arabidopsis shoots utilizing the Agilent DNA array of 21,000 Arabidopsis genes.
