Sentence examples for inner gene from inspiring English sources

Nested PCR was performed using inner gene specific primers located in the previously mentioned regions (Online Resource 4).

Details concerning the nucleotide sequences of the 5′ outer and inner adapter sequences as well as 3′ outer and inner gene specific primers are given in the Additional file 5. PCR products generated using the 5′ inner adapter and 3′ inner gene specific primers were sequenced by Sanger sequencing using the 3′ inner gene specific primer.

In the inner gene clustering, initially the root node is created and then the 4 clustering elements that are selected randomly from the first type gene expression data.

Outer and inner gene specific primers for both 5′ and 3′ RACE were designed based on the partial sequences obtained above.

The outer and inner gene-specific primers were listed in Additional file 13.

A second PCR analysis was performed using the inner primer towards the ligated adaptor sequence (provided in RLM-RACE kit, Ambion) and the inner gene-specific primer (table 1) designed downstream of the predicted stop codon for svPINK1 (see Additional file 4).

L in was the number of links between inner genes, G in was the number of inner genes with links to other inner genes, L out was the number of links between G in inner genes and outer genes.

Thirty more cycles of PCR were further carried out using the inner 5' RNA adaptor primer supplied in the kit and inner 3' gene-specific primer.

All the genes in a functional module were designated inner genes, and those outside a functional module were designated outer genes.

We also found putative alternative promoters σ70 promoters (i.e., promoters associated to inner genes of operons), but with a significantly lower score of prediction (Additional File 10).

G out was the number of outer genes with links to G in inner genes, If C in was greater than C out, we recognized the functional module as compact.